TABLE 1.
Effect of cultivation conditions on OA productiona
| Medium | Cultivation method | Cultivation time (h) | OA concn (μmol/liter) | Yield (%) |
|---|---|---|---|---|
| M9 | A | 88 | 12 ± 5 | |
| M9 plus 1 mM each Glu, 2-AB, and Gly | A | 88 | 212 ± 5 | 21 |
| M9 plus 1 mM each Gln, 2-AB, and Gly | A | 88 | 186 ± 7 | 19 |
| M9 plus 1 mM each Glu, 2-AB, and Gly | B | 24 | 200 ± 3 | 20 |
| M9 plus 1 mM each Glu, 2-AB, and Gly (without shaking) | B | 24 | 39 ± 3 | 3.9 |
| M9 plus 1 mM each Glu and Gly | B | 24 | 10 ± 1 | 1.0 |
| 2.5× M9 plus 2.5 mM each Glu, 2-AB, and Gly | B | 24 | 376 ± 60 | 15 |
| M9 plus 5 mM each Glu, 2-AB, and Gly | B | 24 | 281 ± 36 | 5.6 |
| M9 plus 5 mM each Glu, 2-AB, and Gly | B | 64 | 331 ± 17 | 6.6 |
a
ΔyggSΔggt-GshA*B cells were grown by cultivation method A or B with M9 medium (or 2.5× M9 medium) containing Cam, IPTG, and the indicated concentrations of amino acids. The total amount of OA in the cultivation system was determined at the indicated cultivation times. Yield indicates the level of OA production based on the concentrations of the added amino acids. All results are expressed as means ± standard deviation of the data obtained from at least 3 independent experiments.