TABLE 2.

Concentrations of OA and amino acids after cultivation^a

Component	Concn (μmol/liter)
	ΔyggS Δggt strain	ΔyggS Δggt ΔridA strain	ΔyggS Δggt ΔptsG strain	ΔyggS Δggt ΔmiaA strain
OA	200 ± 3	231 ± 4	182 ± 18	277 ± 7
Glu	8.4 ± 0.2	16 ± 3	0.6 ± 0.8	ND
2-AB	210 ± 24	485 ± 29	3.4 ± 0.7	321 ± 21
Gly	5.6 ± 0.8	525 ± 7	6.3 ± 0.7	583 ± 11

^aThe ΔyggS Δggt, ΔyggS Δggt ΔridA, ΔyggS Δggt ΔptsG, or ΔyggS Δggt ΔmiaA strains, all of which harbored pGshA*B, were grown by cultivation method B with 1 mM each Glu, 2-AB, and Gly. After the 24-h cultivation, the concentrations of OA and amino acids in the cultivation system were determined. Data are expressed as the means ± standard deviations obtained from at least 3 independent experiments. ND, not detected.