Figure 3.

Divergent taxa correlate with FI₃₄ score and chronological age. DESeq2-normalized genus-level OTU abundances are expressed as a heatmap. Taxon abundances are sorted along the x-axis by (A) increasing FI₃₄ score or (B) chronological age. Differential abundance of genus-level OTUs between lower (light orange), middle (middle orange), and upper (dark orange) 33rd percentiles of FI₃₄ or chronological age was calculated using DESeq2. Log₂ fold-change (Log₂ fold ∆) in abundance is expressed as a ratio of the taxon abundance in high-level over low-level FI₃₄ or chronological age. Shades of red fold-change indicate higher abundance in the high group, whereas shades of blue indicate lower abundance in the high group. Group sizes were chosen to normalize sample size between FI₃₄ and chronological age groups (FI₃₄: low n = 30, middle n = 27, high n = 28; Age: low n = 32, middle n = 29, high n = 24). OTUs were filtered for prevalence in at least 25% of subjects. Significant differences in OTU abundances were observed between the lower and upper levels of FI₃₄ scores but not chronological age after correction for multiple comparisons by false-discovery rate (FDR; q < 0.1 above horizontal red bar, p < .05 above the horizontal blue bar). Taxa are sorted down the y-axis by increasing p values. Low FI₃₄ scores ranged from 0 to 0.083, middle scores ranged from 0.091 to 0.137, and high scores ranged from 0.142 to 0.365. Supplementary Figures 3 and 4 contain expanded heatmaps for the results of all tested genera sorted by increasing FI₃₄ score or chronological age, respectively. “*” indicates substitution of higher clade name in lieu of unassigned genus.