Table 2.
Apparent KD and Bmax of Aptamers on PDAC Cell Lines
| Apparent KD (nM) |
Bmax (Relative Units) |
|||
|---|---|---|---|---|
| Waz | E07 | Waz | E07 | |
| Panc-1 | 170 ± 59 | 51 ± 22 | 10 ± 1.2 | 8.1 ± 0.87 |
| MIA PaCa-2 | 160 ± 42 | 26 ± 20 | 14 ± 1.3 | 4.9 ± 0.79 |
| BxPC3 | 110 ± 66 | 67 ± 16 | 12 ± 2.4 | 16 ± 1.0 |
| B16 | >1,000 | >1,000 | 0.89 ± 0.10 | 0.83 ± 0.10 |
Aptamer dose-response curves were used to determine the apparent KD for Waz and E07 on Panc-1, MIA PaCa-2, BxPC3, and B16 cells. Apparent KD values are shown in nanomolars, Bmax values are shown in relative units, and both were calculated based on a plot of the median fluorescence normalized to the fluorescence of C36 to account for nonspecific uptake. Results are based on a minimum of three independent experiments.