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Conformational dynamics observed for spFRET on a TIRF microscope.
| Construct | Acceptor fluorophore | Number of dynamic molecules | Number of static molecules | Dynamic molecules [%] |
|---|---|---|---|---|
| RRM1-2-C187-C318 | Atto647N | 129 | 313 | 29 |
| RRM1-2-C187-C318 + U9 |
Atto647N | 35 | 135 | 21 |
| RRM1-2-C187-C318 | Alexa647 | 3 | 234 | 1 |
| RRM1-2-C187-C318 + U9 |
Alexa647 | 5 | 90 | 5 |
