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. 2017 Dec 30;10:331–348. doi: 10.1016/j.omtn.2017.12.014

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© 2017 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

FOXP1/FOXP2 Regulated the Angiogenesis of GECs by Activating AGGF1 Expression

(A and B) FOXP1 (A) and FOXP2 (B) regulated the viability of GECs. Values are means ± SD (n = 5, each group). **p < 0.01 versus the FOXP1/FOXP2 (+) NC group; ^##p < 0.01 versus the FOXP1/FOXP2 (−) NC group. (C and D) FOXP1 (C) and FOXP2 (D) regulated the migration of GECs. Values are means ± SD (n = 5, each group). **p < 0.01 versus the FOXP1/FOXP2 (+) NC group; ^##p < 0.01 versus the FOXP1/FOXP2 (−) NC group. Scale bar represents 30 μm. (E and F) FOXP1 (E) and FOXP2 (F) regulated the tube formation of GECs (black arrows, tube structures; gray arrows, tube branches). Values are means ± SD (n = 5, each group). *p < 0.05, **p < 0.01 versus the FOXP1/FOXP2 (+) NC group; ^#p < 0.05, ^##p < 0.01 versus the FOXP1/FOXP2 (−) NC group. Scale bar represents 30 μm. (G and H) Schematic representation of human AGGF1 promoter region in 3,000 bp upstream or 200 bp downstream of transcription start site (designated as +1). ChIP PCR products for putative FOXP1 (G) and FOXP2 (H) binding sites and an upstream region not expected to associate with FOXP1/FOXP2 are depicted with bold lines. (I and J) qRT-PCR and western blot detection of FOXP1 regulated the mRNA (I) and protein (J) expressions of AGGF1. GAPDH was used as an endogenous control. Values are means ± SD (n = 5, each group). **p < 0.01 versus FOXP1 (+) NC group; ##p < 0.01 versus FOXP1 (−) NC group. IDV, integrated density value. (K and L) qRT-PCR and western blot detection of FOXP2 regulated the mRNA (K) and protein (L) expressions of AGGF1. GAPDH was used as an endogenous control. Values are means ± SD (n = 5, each group). **p < 0.01 versus FOXP2 (+) NC group; ##p < 0.01 versus FOXP2 (−) NC group. IDV, integrated density value.