Table 2.
Primers and Probes Used for qRT-PCR
| Primer or Probe | Gene | Sequence (5′→3′) or Assay ID |
|---|---|---|
| Primer | circ-SHKBP1 | F: AGGTCAGGCAGAGGAAGTCA |
| R: CGCGTCATAACTGGTGATGG | ||
| P: FAM+TCAGTGTCTACCTCACCCCCAAGACC+BHQ1 | ||
| GAPDH | F: GGACCTGACCTGCCGTCTAG | |
| R: TAGCCCAGGATGCCCTTGAG | ||
| P: FAM+CCTCCGACGCCTGCTTCACCACCT+Eclipse | ||
| SHKBP1 | F: ATCTGAATGTGGGAGGCAAG | |
| R: CTCCGGTCTCATCTTTCAGC | ||
| FOXP1 | F: AACCATCAGCCCTCTAGGAGT | |
| R: TTAGGCTGACACACAGGTCC | ||
| FOXP2 | F: TCAAAGCCACCATCAGACAA | |
| R: GACGCCAAAACAATCACAGA | ||
| AGGF1 | F: ATGAGGATGGTTTGCCTGTC | |
| R: TGGAGAAGATGGGTTGGAAG | ||
| Probe | miR-544a | 001138 (Applied Biosystems) |
| miR-379 | 4427975 (Applied Biosystems) | |
| U6 | 001973 (Applied Biosystems) |