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Primers Used for ChIP Experiments
| Gene | Binding Site or Control | Sequences (5′→3′) | Product Size (bp) | Annealing Temperature (°C) |
|---|---|---|---|---|
| AGGF1 | PCR1 | F: AGAGCATCATAGTCCCCACAAA | 165 | 58.2 |
| R: GAAGCCCTAAGAGCGACAGG | ||||
| PCR2 | F: GGCCAATCTACCAGGGACTC | 139 | 61.9 | |
| R: TTCCTGCCTCTCTATGGGACC | ||||
| PCR3 | F: TGGCAAGGGAGAAATGTGACT | 128 | 58 | |
| R: ATGAGGCACCATACCCCTCA | ||||
| PCR4 | F: GCATCACAGGGGACATACCA | 217 | 59.8 | |
| R: CTTGTGCACTGCCCACTAGA |
