Figure 1.

GLP2 Inhibits Osteosarcoma Carcinogenesis In Vitro

(A) The photography of the HEK293 cell lines infected with rLV-Green-GLP2. (B) The photography of the MG63 cell lines infected with rLV-Green or rLV-Green-GLP2. (C) Western blotting with anti-GLP2 in MG63 infected with rLV-GLP2 or rLV. β-actin was used as an internal control. (D) Co-Immunoprecipitation (coIP) with anti-GLP2R followed by western blotting with anti-GLP2 in MG63 cells infected with rLV or rLV-GLP2. IgG IP as negative control. INPUT refers to western blotting with anti-GLP2 and anti-GLP2R. (E) Cell proliferation assay was performed in 96-well format using the CCK8 cells proliferation kit to determine the cell viability as described by the manufacturer. Each sample was assayed in triplicates for 3 days consecutively.Cell growth curve was based on the corresponding the relative values of OD450 and each point represents the mean of three independent samples. Data are means of value from three independent experiments (error bar ± SEM; *p < 0.05). (F) Cell BrdU assay. Data are means of value from three independent experiment (error bar ± SEM; **p < 0.01). (G) (left)The photography of colonies from the cell lines indicated in left. (right) Cell plate colony formation ability assay. Data are means of value from three independent experiment, bar ± SEM. (H) Cell transwell assay. Data are means of value from three independent experiment (error bar ± SEM; **p < 0.01).