Figure 5.

GLP2 Promotes the Directed Differentiation from MG63 to Osteoblast (OB)

(A) The schematic digram illustrates a model of osteoblast induced from human osteosarcoma cell line MG63 with dexamethasone,β- glycerophosphate and mlascorbic acid(Asc) for 21days. (B) Western blotting with anti-GLP2 in induced OB. β-actin as internal control. (C, left) The photography of BALP staining. (C, right). The BALP positive rate. (D, left) The photography of calcific nodules after induction of OB. (D, right) The rate of calcific nodules in OB. (E) RT-PCR analysis for BALP, Ocn and PICP in induced OB.β-actin as internal control. (F) Western blotting with anti-BALP, anti-Ocn, anti-PICP in induced OB. β-actin was used as an internal control. (G) Cell BALP activity assay. Data are means of value from three independent experiment (error bar ± SEM; **p < 0.01). (H) Cell PICP activity assay. Data are means of value from three independent experiment (error bar ± SEM; **p < 0.01).