Figure 6.

GLP2 Promotes the Expression of BMP Dependent on c-Fos

(A) RNA immunoprecipitation (RIP) with anti-METTL3 followed by RT-PCR with c-Fos primers in OB cells induced from MG63 infected with rLV-GLP2 or rLV, respectively. IgG RIP as negative control. RT-PCR for c-Fos as INPUT. (B) RNA Immunoprecipitation(RIP) with anti-m6A followed by RT-PCR with c-Fos primers in OB cells induced from MG63 infected with rLV-GLP2 or rLV, respectively. IgG RIP as negative control. RT-PCR for c-Fos as INPUT. (C) RT-PCR analysis of Fos in OB cells induced from MG63 infected with rLV-GLP2 or rLV, respectively. β-actin as internal control. (D) Western blotting with anti-FOS in OB cells induced from MG63 infected with rLV-GLP2 or rLV, respectively. β-actin was used as an internal control. (E) ChIP assay with anti-FOS followed by PCR with DNA primers of BMP in OB cells induced from MG63 infected with rLV-GLP2 or rLV, respectively. IgG ChIP was the negative control. (F) The assay of luciferase activity of promoter of BMP in OB induced from MG63 infected with rLV-GLP2 or rLV, respectively. **p < 0.01. (G) Western blotting with anti-BMP and RT-PCR with BMP cDNA primers in OB cells induced from MG63 infected with rLV-GLP2 or rLV, respectively. β-actin as internal control.