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. Author manuscript; available in PMC: 2018 Mar 21.
Published in final edited form as: Talanta. 2016 Nov 19;164:154–158. doi: 10.1016/j.talanta.2016.11.039

Fig. 1.

Fig. 1

(A) SDS-PAGE analysis of Nb28; (B) Western blot analysis of Nb28. Blots were stained with SYPRO Ruby protein gel stain or incubated with rabbit anti-6×his tag IgG/HRP conjugate and TMB membrane peroxidase substrate. Lane M: PageRuler unstained protein ladder (A) and spectrum multicolor broad-range protein ladder (B). Lane 1: Whole-cell extract under noninduced conditions. Lane 2: Whole-cell extract under induced conditions. Lane 3: Nb28 purified by high-capacity nickel IMAC resin.