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. Author manuscript; available in PMC: 2018 Mar 21.
Published in final edited form as: Talanta. 2016 Nov 19;164:154–158. doi: 10.1016/j.talanta.2016.11.039

Fig. 3.

Fig. 3

Binding kinetics of Nb15 to the OTA-OVA conjugate. Nbs (25 μg/mL) were immobilized onto the Ni-NTA biosensor (Cat. No.: 18-5101, ForteBio, CA) tips through the 6 × His tag. Then the probe was incubated with OTA-OVA conjugate at different concentrations (3, 10, 30, 90 nM) to determine the corresponding association and dissociation profiles of Nbs. Interferometry data were globally fit to a 1:1 binding model calculating the rate constants (ka and kd) and equilibrium dissociation constant KD (KD = kd/ka). The embedded table includes the value of ka, kd, KD for nanobodies (Nb15, Nb28, Nb32, Nb36) to the OTA-OVA conjugate.