Table 1.
Recoveries of OTA from the spiked cereal samples by Nb-ELISA
| Sample matrix | OTA spiked (μg/kg) | Mean ± SD (μg/kg) | Recovery (%) | RSD (%) |
|---|---|---|---|---|
| Intra-assay (n = 3)a | ||||
| rice | 5 | 5.12 ± 0.18 | 102 | 4 |
| 15 | 12.17 ± 0.15 | 81 | 1 | |
| 25 | 21.32 ± 0.96 | 85 | 5 | |
| oats | 5 | 5.42 ± 0.25 | 108 | 5 |
| 15 | 13.13 ± 0.43 | 88 | 3 | |
| 25 | 20.05 ± 0.81 | 80 | 4 | |
| barley | 5 | 5.24 ± 0.15 | 105 | 3 |
| 15 | 12.44 ± 0.93 | 83 | 8 | |
| 25 | 20.44 ± 1.77 | 82 | 9 | |
| Inter-assay (n = 6)b | ||||
| rice | 5 | 4.97 ± 0.32 | 99 | 6 |
| 15 | 12.77 ± 0.67 | 85 | 5 | |
| 25 | 21.53 ± 1.02 | 86 | 5 | |
a
Each assay was performed in three replicates on the same day.
b
The assays were performed on six different days.