Figure 4.

Dietary δ-T reduced the levels of pAKT in prostate of Pten^p−/− mice. Prostates of the WT mice fed an AIN93M and Pten^p−/− mice fed either an AIN93M or a 0.2% δ-T diet were analyzed for pAKT (T308) levels using IHC staining. Images of representative IHC staining for these mouse prostate samples at the age of 12, 25 and 40 weeks are shown (A–I). The scale bar represents 50 µm. The quantified results of pAKT (T308) levels for the three groups of mice were determined using the Aperio ScanScope and are summarized in (J). Data are presented as mean ± SD (the number of WT mice is six for each time point; the number of Pten^p−/− mice on the AIN93M diet is 7, 7 or 9 for 12, 25 or 40 weeks, respectively and the number of Pten^p−/− mice on the 0.2% δ-T diet is 7, 7 or 10 for 12, 25 or 40 weeks, respectively). *P = 0.033, **P = 0.017 and ***P = 0.023.