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. 2017 Oct 11;39(2):134–145. doi: 10.1093/carcin/bgx114

Figure 5.

Figure 5.

BITC administration inhibits multiplicity of skeletal metastasis induced by intra-cardiac injection of luciferase expressing MDA-MB-231 cells in female nude mice. (A) Bioluminescence and bone CT images of control- and BITC-treated mice 8 weeks after cell injection. Bar at right indicates intensity of bioluminescence signal (red is highest and blue is lowest). Yellow arrows and red dotted circle exemplify bone metastasis and osteolytic bone erosion, respectively. (B) Longitudinal bioluminescence (left panel) and CT (right panel) imaging of a control mouse (mouse # B11L) and a BITC-treated mouse (mouse # B3N) after intra-cardiac injection of cells. (C) Incidence of bone metastases in control- and BITC-treated mice as determined by bioluminescence and CT. P value was determined by Fisher’s exact test (n = 9 for both groups). (D) Multiplicity of bone metastasis per mouse in control- and BITC-treated mice at the end of study. Columns indicate average, and bars indicate standard deviation. P value was determined by unpaired t-test (n = 9 for both groups). (E) Representative hematoxylin and eosin-stained bone section (×200 magnification) from a control mouse (mouse # B21L) and a BITC-treated mouse (mouse # B3N). Dotted circle indicates bone erosion in mouse # B21L.