Figure 5.

RAD51AP1 was aberrantly expressed—primarily in the nucleus—in both SKOV3 (A, B) and A549 cells (C, D), as it was evident by ImageStream (A, C) and immunofluorescent analyses (B, D). Using siRNA for RAD51AP1, a substantial and significant downregulation of the gene was evident at both 48 and 72 h post transfection for both SKOV3 (E) and A549 (F) cells compared with scrambled control. The downregulation was mirrored at protein level with maximal downregulation at 72 h post transfection (G). Silencing of RAD51AP1 resulted in significant inhibition of cell growth at 48 and 72 h for SKOV3 cells compared with control untransfected and scrambled siRNA cells (H), whereas in A549 cells a significant decrease in cell proliferation was also evident only at 72 h (I). **P < 0.01, ***P < 0.001. Magnification ×40.