Figure 1.

Generation of ARH1 and CD38 double-knockout mice. (A) Genotyping of mice. ARH1 primers amplified a 0.8-kb fragment from the wild-type allele and a 1.0-kb fragment from the knockout allele (solid arrow). CD38 primers amplified a 0.4-kb fragment from the wildtype allele and a 0.5-kb fragment from the knockout allele (dotted arrow). Two mice were used for each genotype. (B) CD38 expression by splenocytes from wild-type, ARH1^−/−, CD38^−/− and ARH1^−/−/CD38^−/− mice as assessed by flow cytometry. Data are representative of two independent experiments and are shown as histograms with an overlay of CD38 expression by wild-type splenocytes (gray peak). (C) ARH1 expression in mouse brain lysates detected by Western blot (n = 3).