Table 1. Agents administered to mice.
Agent | Company | Injection route | Volume | Concentration (Vehicle) | N of mice |
---|---|---|---|---|---|
Non-fluorescent phalloidin | Merck Millipore | Intra-vitreous | 2 μl | 5 µg/µl (water) | 4 |
Fluorescent phalloidin | Biotium | Intra-vitreous | 2 μl | 200 U/ml (water) | 15 |
Jasplakinolide | Abcam | Intra-vitreous | 2 μl | 10 μM (saline) | 3 |
Scrambled (control) siRNA | Ambion LifeTech | Intra-vitreous | 3 μl | 0.5 mg/ml (10% Glucose, nuclease free water, in vivo-jetPEI®) |
9 |
α-SMA-siRNA | Ambion LifeTech | Intra-vitreous | 3 μl | 0.5 mg/ml (10% Glucose, nuclease free water, in vivo-jetPEI®) |
9 |
BLOCK-iTTM Alexa Fluor® 555 Fluorescent control |
Thermo Fisher Scientific | Intra-vitreous | 3 μl | 0.5 mg/ml (10% Glucose, nuclease free water, in vivo-jetPEI®) |
2 |