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. 2018 Mar 21;7:e26148. doi: 10.7554/eLife.26148

Table 9. List of oligonucleotide sequences of molecules listed in the manuscript.

Northern probes
miR-450b-5p 5′-TAT TCA GGA ACA TAT TGC AAA A-3′
miR-495 5′-AAG AAG TGC ACC ATG TTT GTT T-3′
miR-30e 5′-CTT CCA GTC AAG GAT GTT TAC A-3′
miR-330–3 p 5′-TCT CTG CAG GCC GTG TGC TTT GC-3′
miR-382 5′-CGA ATC CAC CAC GAA CAA CTT C-3′
miR-423–3 p 5′-ACT GAG GGG CCT CAG ACC GAG CT-3′
miR-135a 5′-TCA CAT AGG AAT AAA AAG CCA TA-3′
miR-144 5′-AGT ACA TCA TCT ATA CTG TA-3′
miR-301b 5′-GCT TTG ACA ATA TCA TTG CAC TG-3′
miR-590–3 p 5′-ACT AGC TTA TAC ATA AAA TTA-3′
miR-301a 5′-GCT TTG ACA ATA CTA TTG CAC TG-3′
miR-34c-5p 5′-GCA ATC AGC TAA CTA CAC TGC CT-3′
RT-PCR
EIF5A2
 FW 5′-ATG GCA GAC GAA ATT GAT TTC ACT A-3′
 RV 5′-CTC ATT GCA CAC ATG ACA GAC-3′
SCA1
 FW 5′-ACG GTC ATT CAG ACC ACA CA-3′
 RV 5′-CAG GGT TGA AGT TCT CGC TC-3′
ITGB1
 FW 5′-CGC CGC GCG GAA AAG ATG AAT-3′
 RV 5′-TGG GCT GGT GCA GTT CTG TTC A-3′
c-RAF
 FW 5′-CGA CCC ACA GTG GAC GAT CCA G-3′
 RV 5′-AGA TAA TGC TGG CCG ACT GGC CT-3′
MEK1
 FW 5′-AAG GGA ATC CCG GGC TGC CGA A-3′
 RV 5′-GCC ATC GCT GTA GAA CGC ACC A-3′
MAPK
 FW 5′-GCA CCG TGA CCT CAA GCC TTC-3′
 RV 5′-CAC CGA TGT CTG AGC ACG TCC AG-3′
LAMA3
FW 5′-GAT GGC TCA GGC ATA TGT GTT-3′
RV 5′-CTG GCC ATT GCT GTT ACA ACT-3′
TUBA
 FW 5′-TGA CCT GAC AGA ATT CCA GAC CA-3′
 RV 5′-GCA TTG ACA TCT TTG GGA ACC AC-3′
shRNA (target sequence underlined; BamH1/EcoR1 cohesive ends italicized)
EIF5A2sh
 Sense 5′-GAT CCG CTG CCA GAA GGT GAA CTA GCT TCC TGT CAG ATA TAT CTC TCC TTC CAC ACT TTT TG-3′
 Antisense 5′-AAT TCA AAA ACT GCC AGA AGG TGA ACT AGT CTG ACA GGA AGT ATA TCT CTC CTT CCA CAC G-3′
ScaIsh
 Sense 5′-GAT CCG AAC CTG AAG AAC GGC TCT CTT CCT GTC AGA AGA GCC GTT CTT CAG GTT CTT TTT G-3′
 Antisense 5′-AAT TCA AAA AGA ACC TGA AGA ACG GCT CTT CTG ACA GGA AGA GAG CCG TTC TTC AGG TTC G-3′
p65sh
 Sense 5′-GAT CCG GAC ATA TGA GAC CTT CAA CTT CCT GTC AGA TTG AAG GTC TCA TAT GTC CTT TTT G-3′
 Antisense 5′-AAT TCA AAA AGG ACA TAT GAG ACC TTC AAT CTG ACA GGA AGT TGA AGG TCT CAT ATG TCC G-3′
p50/p100sh
 Sense 5′-GAT CCG AGC TAA TCC GCC AAG CAG CTT CCT GTC AGA CTG CTT GGC GGA TTA GCT CTT TTT G-3′
 Antisense 5′-AAT TCA AAA AGA GCT AAT CCG CCA AGC AGT CTG ACA GGA AGC TGC TTG GCG GAT TAG CTC G-3′
LAMA3sh
 Sense 5′-GAT CCG GAG TCC TTC TGG ATT ACC CTT CCT GTC AGA GGT AAT CCA GAA GGA CTC CTT TTT G-3′
 Antisense 5′-AAT TCA AAA AGG AGT CCT TCT GGA TTA CCT CTG ACA GGA AGG GTA ATC CAG AAG GAC TCC G-3′
Overexpressing constructs
HOXD10
 FW 5′-CGG CAG GCG CGC CGC CAC CAT GTC CTT TCC CAA CAG CTC TCC T-3′ (AscI site italicized)
 RV 5′-CCG GCC GAA TTC CTA AGA AAA CGT GAG GTT GGC GGT CAG-3′ (EcoR1 site italicized)
p53
 FW 5’-GAT CTC GGA TCC GCC ACC ATG GAG GAG CCG CAG TCA GAT CCT AGC-3’ (BamH1 site italicized)
 RV 5′-TAC AGG AAT TCT CAG TCT GAG TCA GGC CCT TCT GTC TTG AAC ATG-3′ (EcoR1 site italicized)
ATXN1 and EIF5A2
 FW 5′-TCT AGA GCC CGG GCG CGC CGG CCG CCG CGA TCG CCA TG-3′
 RV 5-’′GCA GAT CCT TCG CGG CCG CGT TAA ACC TTA TCG TCG TCA TCC TTG TAA TCC AGG ATA TCA TTT GC-3′
miRNA reporter constructs (Mlu1/Xho1 sites italicized)
miR-34c
3–0 kb
 FW 5′-GAC TAC GCG TAC CGC TGG CAG TTC ATT TTA GCT C-3′ (Mlu1 site italicized)
 RV 5′-GAC TCT CGA GCT AGA AGA TGG AGG CCC AGA TTC TTG AGA C-3′ (Xho1 site italicized)
2–0 kb
 FW 5′-GAC TAC GCG TCT TGG CTT CCT CCT AGT CAT CAA CCT-3′ (Mlu1 site italicized)
 RV 5′-GAC TCT CGA GTC TGA TCT AGC AGG AGG GAC AAA GAG-3′ (Xho1 site italicized)
1–0 kb
 FW 5′-GAC TAC GCG TTC CCT TCA CTA TGG GGT GTA CAG AAC-3′ (Mlu1 site italicized)
 RV 5′-GAC TCT CGA GCT AGA AGA TGG AGG CCC AGA TTC TTG AGA C-3′ (Xho1 site italicized)
3–2 kb
 FW 5′-GAC TAC GCG TTT ATA AAA ACC GCT GGC AGT TCA TTT TAG C-3′ (Mlu1 site italicized)
 RV 5′-GAC TCT CGA GAG GAG GAA GCC AAG AAG AGT GTA GAA AAC A-3′ (Xho1 site italicized)
2–1 kb
 FW 5′-GAC TAC GCG TCT ATT CTC CCA CCT CAG CC TCC AAG TAG-3′ (Mlu1 site italicized)
 RV 5′-GAC TCT CGA GCT GTA CAC CCC ATA GTG AAG GGA AAG AAA C-3′ (Xho1 site italicized)
miR-30e
3–0 kb
 FW 5′-GAC TAC GCG TGC CAC CAT GCC CGG CTA A-3′ (Mlu1 site italicized)
 RV 5′-GAC TCT CGA GGG GAG CTC GAG ATC TGA GTT TTG ACC-3′ (Xho1 site italicized)
2–0 kb
 FW 5′-GAC TAC GCG TCT GGT CTT GAA CTC CTG ACC TCG TCA T-3′ (Mlu1 site italicized)
 RV 5′-GAC TCT CGA GTT CGG GAG CTC GAG ATC TGA GTT TTG-3′ (Xho1 site italicized)
1–0 kb
 FW 5′-GAC TAC GCG TTT AGA TCT GGG TAC AGA TGA AGG AAT TGA GAC TCC-3′ (Mlu1 site italicized)
 RV 5′-GAC TCT CGA GTT CGG GAG CTC GAG ATC TGA TGG TTG-3′ (Xho1 site italicized)
3–2 kb
 FW 5′-GAC TAC GCG TCT TTT TGA ACT CCA GCA GCA CAT GAA CTA T-3′ (Mlu1 site italicized)
 RV 5′-GAC TCT CGA GGG CCT TGT TTT GAC CAA TGA AAT ATG AGT A-3′ (Xho1 site italicized)
2–1 kb
 FW 5′-GAC TAC GCG TCT GGT CTT GAA CTC CTG ACC TCG TCA T-3′ (Mlu1 site italicized)
 RV 5′-GAC TCT CGA GAC ACT TGA CTT CAG GGA GTC TCA ATT CCT T-3′ (Xho1 site italicized)
miR-144
3–0 kb
 FW 5′-GAC TAC GCG TCT CAC TAT AAG ACT CGG GCC AAG CAC TTC-3′ (Mlu1 site italicized)
 RV 5′-GAC TCT CGA GGC CAG TTG TGG TGG CAT GTG-3′ (Xho1 site italicized)
2–0 kb
 FW 5′-GAC TAC GCG TGT TGC CCA GGC TGG AGT ACA ATA GGA T-3′ (Mlu1 site italicized)
 RV 5′-GAC TCT CGA GAA TTA GCC AGT TGT GGT GGC ATG TG-3′ (Xho1 site italicized)
1–0 kb
 FW 5′-GAC TAC GCG TGT ACT GGG GAG GCA GAG GAA TGG AAG-3′ (Mlu1 site italicized)
 RV 5′-GAC TCT CGA GAA TTA GCC AGT TGT GGT GGC ATG TG-3′ (Xho1 site italicized)
3–2 kb
 FW 5′-GAC TAC GCG TCC TAT TCC TAG CGG GTT TGT GCA TAG AG-3′ (Mlu1 site italicized)
 RV 5′-GAC TAG ATC TCT GGG CAA CAA GAG CAA AAC TGG ATC-3′ (Bgl1I site italicized)
2–1 kb
 FW 5′-GAC TAC GCG TCC CAG GCT GGA GTA CAA TAG GAT GAT CT-3′ (Mlu1 site italicized)
 RV 5′-GAC TCT CGA GGC CCA GGG CTG TTT TCC TGG ATA TT-3′ (Xho1 site italicized)
ChIP
miR-34c (−1~0 kb)
 FW 5′-GTG TCA GCA ATG GGT GCT CTA-3′
 RV 5′-CCA GAG GAG GTG AGA CTT GAG-3′
miR-30e (−3~−2 kb)
 FW 5′-GAG GCA GTC TGA GAT ATT CCC-3′
 RV 5′-CTG CAG CAT AAC ATG CTA GCT-3′
miR-144 (−3~−2 kb)
 FW 5′-CTG TGA TGA GGA CAA CAG TAA-3′
 RV 5′-ATC CCC CTA CCT CAG CCT CTC-3′