| miR-450b-5p |
5′-TAT TCA GGA ACA TAT TGC AAA A-3′ |
| miR-495 |
5′-AAG AAG TGC ACC ATG TTT GTT T-3′ |
| miR-30e |
5′-CTT CCA GTC AAG GAT GTT TAC A-3′ |
| miR-330–3 p |
5′-TCT CTG CAG GCC GTG TGC TTT GC-3′ |
| miR-382 |
5′-CGA ATC CAC CAC GAA CAA CTT C-3′ |
| miR-423–3 p |
5′-ACT GAG GGG CCT CAG ACC GAG CT-3′ |
| miR-135a |
5′-TCA CAT AGG AAT AAA AAG CCA TA-3′ |
| miR-144 |
5′-AGT ACA TCA TCT ATA CTG TA-3′ |
| miR-301b |
5′-GCT TTG ACA ATA TCA TTG CAC TG-3′ |
| miR-590–3 p |
5′-ACT AGC TTA TAC ATA AAA TTA-3′ |
| miR-301a |
5′-GCT TTG ACA ATA CTA TTG CAC TG-3′ |
| miR-34c-5p |
5′-GCA ATC AGC TAA CTA CAC TGC CT-3′ |
| RT-PCR |
| EIF5A2 |
| FW |
5′-ATG GCA GAC GAA ATT GAT TTC ACT A-3′ |
| RV |
5′-CTC ATT GCA CAC ATG ACA GAC-3′ |
| SCA1 |
| FW |
5′-ACG GTC ATT CAG ACC ACA CA-3′ |
| RV |
5′-CAG GGT TGA AGT TCT CGC TC-3′ |
| ITGB1 |
| FW |
5′-CGC CGC GCG GAA AAG ATG AAT-3′ |
| RV |
5′-TGG GCT GGT GCA GTT CTG TTC A-3′ |
| c-RAF |
| FW |
5′-CGA CCC ACA GTG GAC GAT CCA G-3′ |
| RV |
5′-AGA TAA TGC TGG CCG ACT GGC CT-3′ |
| MEK1 |
| FW |
5′-AAG GGA ATC CCG GGC TGC CGA A-3′ |
| RV |
5′-GCC ATC GCT GTA GAA CGC ACC A-3′ |
| MAPK |
| FW |
5′-GCA CCG TGA CCT CAA GCC TTC-3′ |
| RV |
5′-CAC CGA TGT CTG AGC ACG TCC AG-3′ |
| LAMA3 |
| FW 5′-GAT GGC TCA GGC ATA TGT GTT-3′ |
| RV 5′-CTG GCC ATT GCT GTT ACA ACT-3′ |
| TUBA |
| FW |
5′-TGA CCT GAC AGA ATT CCA GAC CA-3′ |
| RV |
5′-GCA TTG ACA TCT TTG GGA ACC AC-3′ |
|
shRNA (target sequence underlined; BamH1/EcoR1 cohesive ends italicized) |
| EIF5A2sh |
| Sense |
5′-GAT CCG CTG CCA GAA GGT GAA CTA GCT TCC TGT CAG ATA TAT CTC TCC TTC CAC ACT TTT TG-3′ |
| Antisense |
5′-AAT TCA AAA ACT GCC AGA AGG TGA ACT AGT CTG ACA GGA AGT ATA TCT CTC CTT CCA CAC G-3′ |
| ScaIsh |
| Sense |
5′-GAT CCG AAC CTG AAG AAC GGC TCT CTT CCT GTC AGA AGA GCC GTT CTT CAG GTT CTT TTT G-3′ |
| Antisense |
5′-AAT TCA AAA AGA ACC TGA AGA ACG GCT CTT CTG ACA GGA AGA GAG CCG TTC TTC AGG TTC G-3′ |
| p65sh |
| Sense |
5′-GAT CCG GAC ATA TGA GAC CTT CAA CTT CCT GTC AGA TTG AAG GTC TCA TAT GTC CTT TTT G-3′ |
| Antisense |
5′-AAT TCA AAA AGG ACA TAT GAG ACC TTC AAT CTG ACA GGA AGT TGA AGG TCT CAT ATG TCC G-3′ |
| p50/p100sh |
| Sense |
5′-GAT CCG AGC TAA TCC GCC AAG CAG CTT CCT GTC AGA CTG CTT GGC GGA TTA GCT CTT TTT G-3′ |
| Antisense |
5′-AAT TCA AAA AGA GCT AAT CCG CCA AGC AGT CTG ACA GGA AGC TGC TTG GCG GAT TAG CTC G-3′ |
| LAMA3sh |
| Sense |
5′-GAT CCG GAG TCC TTC TGG ATT ACC CTT CCT GTC AGA GGT AAT CCA GAA GGA CTC CTT TTT G-3′ |
| Antisense |
5′-AAT TCA AAA AGG AGT CCT TCT GGA TTA CCT CTG ACA GGA AGG GTA ATC CAG AAG GAC TCC G-3′ |
| Overexpressing constructs |
| HOXD10 |
| FW |
5′-CGG CAG GCG CGC CGC CAC CAT GTC CTT TCC CAA CAG CTC TCC T-3′ (AscI site italicized) |
| RV |
5′-CCG GCC GAA TTC CTA AGA AAA CGT GAG GTT GGC GGT CAG-3′ (EcoR1 site italicized) |
| p53 |
| FW |
5’-GAT CTC GGA TCC GCC ACC ATG GAG GAG CCG CAG TCA GAT CCT AGC-3’ (BamH1 site italicized) |
| RV |
5′-TAC AGG AAT TCT CAG TCT GAG TCA GGC CCT TCT GTC TTG AAC ATG-3′ (EcoR1 site italicized) |
| ATXN1 and EIF5A2 |
| FW |
5′-TCT AGA GCC CGG GCG CGC CGG CCG CCG CGA TCG CCA TG-3′ |
| RV |
5-’′GCA GAT CCT TCG CGG CCG CGT TAA ACC TTA TCG TCG TCA TCC TTG TAA TCC AGG ATA TCA TTT GC-3′ |
| miRNA reporter constructs (Mlu1/Xho1 sites italicized) |
| miR-34c |
|
| 3–0 kb |
|
| FW |
5′-GAC TAC GCG TAC CGC TGG CAG TTC ATT TTA GCT C-3′ (Mlu1 site italicized) |
| RV |
5′-GAC TCT CGA GCT AGA AGA TGG AGG CCC AGA TTC TTG AGA C-3′ (Xho1 site italicized) |
| 2–0 kb |
|
| FW |
5′-GAC TAC GCG TCT TGG CTT CCT CCT AGT CAT CAA CCT-3′ (Mlu1 site italicized) |
| RV |
5′-GAC TCT CGA GTC TGA TCT AGC AGG AGG GAC AAA GAG-3′ (Xho1 site italicized) |
| 1–0 kb |
|
| FW |
5′-GAC TAC GCG TTC CCT TCA CTA TGG GGT GTA CAG AAC-3′ (Mlu1 site italicized) |
| RV |
5′-GAC TCT CGA GCT AGA AGA TGG AGG CCC AGA TTC TTG AGA C-3′ (Xho1 site italicized) |
| 3–2 kb |
|
| FW |
5′-GAC TAC GCG TTT ATA AAA ACC GCT GGC AGT TCA TTT TAG C-3′ (Mlu1 site italicized) |
| RV |
5′-GAC TCT CGA GAG GAG GAA GCC AAG AAG AGT GTA GAA AAC A-3′ (Xho1 site italicized) |
| 2–1 kb |
|
| FW |
5′-GAC TAC GCG TCT ATT CTC CCA CCT CAG CC TCC AAG TAG-3′ (Mlu1 site italicized) |
| RV |
5′-GAC TCT CGA GCT GTA CAC CCC ATA GTG AAG GGA AAG AAA C-3′ (Xho1 site italicized) |
| miR-30e |
|
| 3–0 kb |
|
| FW |
5′-GAC TAC GCG TGC CAC CAT GCC CGG CTA A-3′ (Mlu1 site italicized) |
| RV |
5′-GAC TCT CGA GGG GAG CTC GAG ATC TGA GTT TTG ACC-3′ (Xho1 site italicized) |
| 2–0 kb |
|
| FW |
5′-GAC TAC GCG TCT GGT CTT GAA CTC CTG ACC TCG TCA T-3′ (Mlu1 site italicized) |
| RV |
5′-GAC TCT CGA GTT CGG GAG CTC GAG ATC TGA GTT TTG-3′ (Xho1 site italicized) |
| 1–0 kb |
|
| FW |
5′-GAC TAC GCG TTT AGA TCT GGG TAC AGA TGA AGG AAT TGA GAC TCC-3′ (Mlu1 site italicized) |
| RV |
5′-GAC TCT CGA GTT CGG GAG CTC GAG ATC TGA TGG TTG-3′ (Xho1 site italicized) |
| 3–2 kb |
|
| FW |
5′-GAC TAC GCG TCT TTT TGA ACT CCA GCA GCA CAT GAA CTA T-3′ (Mlu1 site italicized) |
| RV |
5′-GAC TCT CGA GGG CCT TGT TTT GAC CAA TGA AAT ATG AGT A-3′ (Xho1 site italicized) |
| 2–1 kb |
|
| FW |
5′-GAC TAC GCG TCT GGT CTT GAA CTC CTG ACC TCG TCA T-3′ (Mlu1 site italicized) |
| RV |
5′-GAC TCT CGA GAC ACT TGA CTT CAG GGA GTC TCA ATT CCT T-3′ (Xho1 site italicized) |
| miR-144 |
|
| 3–0 kb |
|
| FW |
5′-GAC TAC GCG TCT CAC TAT AAG ACT CGG GCC AAG CAC TTC-3′ (Mlu1 site italicized) |
| RV |
5′-GAC TCT CGA GGC CAG TTG TGG TGG CAT GTG-3′ (Xho1 site italicized) |
| 2–0 kb |
|
| FW |
5′-GAC TAC GCG TGT TGC CCA GGC TGG AGT ACA ATA GGA T-3′ (Mlu1 site italicized) |
| RV |
5′-GAC TCT CGA GAA TTA GCC AGT TGT GGT GGC ATG TG-3′ (Xho1 site italicized) |
| 1–0 kb |
|
| FW |
5′-GAC TAC GCG TGT ACT GGG GAG GCA GAG GAA TGG AAG-3′ (Mlu1 site italicized) |
| RV |
5′-GAC TCT CGA GAA TTA GCC AGT TGT GGT GGC ATG TG-3′ (Xho1 site italicized) |
| 3–2 kb |
|
| FW |
5′-GAC TAC GCG TCC TAT TCC TAG CGG GTT TGT GCA TAG AG-3′ (Mlu1 site italicized) |
| RV |
5′-GAC TAG ATC TCT GGG CAA CAA GAG CAA AAC TGG ATC-3′ (Bgl1I site italicized) |
| 2–1 kb |
|
| FW |
5′-GAC TAC GCG TCC CAG GCT GGA GTA CAA TAG GAT GAT CT-3′ (Mlu1 site italicized) |
| RV |
5′-GAC TCT CGA GGC CCA GGG CTG TTT TCC TGG ATA TT-3′ (Xho1 site italicized) |
| ChIP |
| miR-34c (−1~0 kb) |
| FW |
5′-GTG TCA GCA ATG GGT GCT CTA-3′ |
| RV |
5′-CCA GAG GAG GTG AGA CTT GAG-3′ |
| miR-30e (−3~−2 kb) |
| FW |
5′-GAG GCA GTC TGA GAT ATT CCC-3′ |
| RV |
5′-CTG CAG CAT AAC ATG CTA GCT-3′ |
| miR-144 (−3~−2 kb) |
| FW |
5′-CTG TGA TGA GGA CAA CAG TAA-3′ |
| RV |
5′-ATC CCC CTA CCT CAG CCT CTC-3′ |