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. Author manuscript; available in PMC: 2018 Mar 21.
Published in final edited form as: Biochemistry. 2017 Dec 20;57(3):286–294. doi: 10.1021/acs.biochem.7b00874

Figure 2. Quantification analysis of cysteine modification level.

Figure 2

A. Reducing SDS-PAGE serves as an additional purification step after the hVKOR protein is affinity purified. The sliced gel bands (boxes) are used for in-gel isotope labeling and digestion. B. LC/MS/MS analysis of a peptide containing Cys43, which is modified by NEM (green spheres) and NEM-d5 (red). C. Peak areas of the modified peptides are used for calculating percentage of NEM-d5 modification. D. Analysis results are displayed as a bar graph.