Skip to main content
. Author manuscript; available in PMC: 2018 Mar 21.
Published in final edited form as: Biochemistry. 2017 Dec 20;57(3):286–294. doi: 10.1021/acs.biochem.7b00874

Figure 3. Control experiments to identify membrane-buried and disulfide-bonded cysteines.

Figure 3

Cys16 and Cys85 are membrane buried. Cys43, Cys51, Cys132, and Cys135 can form disulfide bonds9. Native membrane: NEM labeling was carried out with intact cell membrane, as described in Special Note 1. Triton X-100: membrane solubilization and NEM labeling were carried out at the same time, as described in the Experimental Protocol. DTT: the cysteines in hVKOR were reduced in cells before the NEM labeling.