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. 2017 Dec 30;10:317–330. doi: 10.1016/j.omtn.2017.12.015

Figure 3.

Figure 3

Detection of H2EH3 Internalization by z Stack Confocal Microscopy and Evaluation of Protein Levels by Western Blot

(A) Cy5-labeled H2EH2 or Cy5-labeled muH2EH3 was added into BT474 cells for 12 hr at 37°C. LysoTracker Green was used to show lysosomes and endosomes. DAPI was used to display nucleus. Confocal laser scanning microscopy with z stack was performed to show cell binding and internalization. Scale bar, 10 μm. (B) Western blot analysis of total HER family receptors and apoptosis-associated molecules in BT474 cells. (C) Western blot analysis of total HER family receptors and apoptosis-associated molecules in SKBR3 cells.