U2 Can Internalize into U87-EGFRvIII Cells
(A) U87-EGFRvIII cells were treated with 2 μM FAM-U2 for 5 and 20 min. Cells were fixed and labeled with anti-EGFR antibody targeting on the cell membrane without permeabilization. Green: fluorescence labeling FAM-U2; blue: cell nucleus (staining by DAPI); red: anti-EGFR antibody. (B) Z stack of U87-EGFRvIII cells incubated with 2 μM FAM-U2 for 30 min. Scale bar, 10 μm. Cells were fixed, permeabilized, and labeled with anti-EGFR and anti-EEA1 antibodies. Green: fluorescence labeling FAM-U2; red: anti-EEA1 antibody; blue: anti-EGFR antibody.