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. 2018 Jan 8;10:438–449. doi: 10.1016/j.omtn.2018.01.001

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© 2018 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

U2 Inhibits the Migration and Invasion of U87-EGFRvIII

(A) U2 inhibits U87-EGFRvIII cell migration obtained by scratch assays to measure cell migration. U87-EGFRvIII cells treated for 8 and 24 hr. Microscopy images were taken at the indicated times. (B) The extent of wound closure was calculated. *p < 0.05 and **p < 0.01. (C) A transwell migration assay was performed in the presence of U2 or GN for 24 and 48 hr. Photographs of a representative experiment are shown. (D) Data from the transwell migration assay are presented as the percentage of migrated cells in the presence of U2 compared with GN control. Each determination represents the average of three individual experiments, and error bars represent the SD. ***p < 0.001 relative to GN. (E) Invasion of U87MG and U87-EGFRvIII cells through matrigel was evaluated in the presence of U2 or GN for the indicated times. Photographs of a representative experiment are shown. (F) Data from the invasion assay are presented as the percentage of invaded cells in the presence of U2 compared with GN control. ***p < 0.001 relative to GN.