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HIV-1 co-receptor usage in tonsil cells from cell-free and cell-associated HIV-1 IIIB (Panel B, D, F and H) and HIV-1 BAL (Panel A, C, E and G) virus after crossing the cervix mucosa on day 12 was detected by simultaneous immunophenotyping and ultrasensitive fluorescence in situ hybridization. Cells were stained with antibodies to CCR5 and CXCR4 markers.
