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. 2016 Aug 22;9(17):13139–13153. doi: 10.18632/oncotarget.11487

Figure 2. siRNA-mediated knockdown reduces cell proliferation in MDA-MB-468 breast cancer cells.

Figure 2

A. PHGDH mRNA levels following 72-120 h siRNA-mediated knockdown in three breast cancer cell lines. mRNA expression of PHGDH was normalised to GAPDH levels. B. Western blot analysis of PHGDH levels in breast cancer cell lines following PHGDH knockdown. C. Cell proliferation measurements determined by colony formation assays. Graph represents averages and standard deviations of three independent experiments with three intra-assay repeats. Data is normalised to the respective scrambled siRNA control.