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. 2018 Jan 10;9(17):13206–13221. doi: 10.18632/oncotarget.24152

Figure 1. Binding of 2448 on two isogenic breast cancer cell line.

Figure 1

The isogenic breast cancer cell lines (MCF7-D10 and MCF7-2101) represent epithelial and mesenchymal phenotypes of EMT. (A) MCF-D10 and MCF7-2101 isogenic cell lines were stained with 2448 and visualized by epifluorescence microscopy. Membranous binding of 2448 was observed on MCF7-D10 cells but not on the isogenic MCF7-2101 cells. The loss of binding on MCF7-2101 corresponded to EMT-like morphological changes. MCF7-D10 are more epithelial with cuboidal or “cobblestone-like” cells whereas MCF7-2101 cells are more mesenchymal-like (ie. more isolated and elongated). (B) High levels of E-cadherin and low levels of the vimentin were expressed in MCF7-D10 cells versus MCF72101 cells. (C) Flow cytometry confirmed binding of 2448 on live MCF7-D10 cells, but not live MCF7-2101 cells. Positive binding is indicated by a rightward shift in fluorescence intensity (shaded) of histogram.