Table 1. Binding of antibody 2448 on the surface of ovarian and breast cell lines.
| Ovarian | mAb clone | Breast | mAb clone | ||
|---|---|---|---|---|---|
| EMT Category† | Cell line | 2448º | EMT Category† | Cell line | 2448º |
| E | CaOV3 | +++ | E | BT474 | ++++ |
| E | OVCAR3 | +++ | E | CAMA1 | ++++ |
| E | OVCAR8 | ++++ | E | HCC2218 | ++++ |
| E | OV90 | +++ | E | MDA453 | ++++ |
| E | IGROV1 | ++++ | E | MCF7 | ++++ |
| IE | OV17R | + | E | SKBR3 | ++++ |
| IE | OVCA432 | ++++ | E | T47D | ++++ |
| IE | OVCA433 | +++ | IE | BT20 | ++++ |
| IM | CH1 | + | IE | HCC1937 | ++++ |
| IM | HEY | – | IM | HCC1954 | ++++ |
| IM | HEYC2 | – | M | MCF10A | ++ |
| IM | SKOV3 | +++ | M | MDA-MB-231 | – |
| IM | SKOV3(D10) | + | M | BT549 | – |
| M | A2780 | – | M | HS578T | ++ |
| M | COLO720E | – | U | 184B5 | ++++ |
| M | HEYA8 | – | U | HCC1395 | – |
| M | OVCAR10 | – | |||
| M | TOV112 | – | |||
| U | IOSE523 | – | |||
Supernatant from hybridoma clones of 2448 was screened by flow cytometry on eighteen ovarian tumor cell lines and one immortalized ovarian surface epithelium cell line, IOSE523 and fourteen breast tumor cell lines and one immortalized mam mary epithelial cell line, MCF10A. Overall, stronger binding was observed on cell lines with an Epithelial or Intermediate Epithelial phenotype. Binding intensity was graded according to binding percentages after marker gating with a 2.0–2.5% cut-off from the negative control: “–” < 10% binding, “+” = 10–40% binding, “++” = 40–60%, “+++” = 60–80% and “++++” > 80%.
†Cell lines were classified according to an Epithelial-Mesenchymal Transition phenotype based on work done by Huang et al. [13] and Akalay et al. [14]: “E” = Epithelial, “IE” = Intermediate Epithelial, “IM” = Intermediate Mesenchymal, “M” = Mesenchymal and “U” = Not Classified. Cell lines 184B5, HCC1395 and IOSE523 were not classified (U).