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. 2018 Jan 30;9(17):13353–13365. doi: 10.18632/oncotarget.24350

Figure 6. Ni(SalPipNONO) has antiangiogenic activities.

Figure 6

(A) A549 cells were treated with Ni(SalPipNONO) 0.5 mM for 24 h and HIF-1α and VEGF protein expression was evaluated by western blot. *p < 0.05 and ***p < 0.001 vs untreated cells. (B) Direct antiagiogenic activity of Ni(SalPipNONO) on cultured endothelial cells. HUVEC were treated with VEGF (20 ng/ml) in the absence/presence of Ni(SalPipNONO) 0.1 mM. The number of cells was counted after 48 h following cell fixation and staining. ***p < 0.001 vs untreated cells. ###p < 0.01 vs VEGF alone. (C) Ni(SalPipNONO) exerts antiangionic activity in vivo. Mice were subcutaneously implanted with Matrigel containing VEGF (300 ng) in the absence or presence of 0.5 mM Ni(SalPipNONO). Plugs were removed after 10 days. Pictures are representative plugs out of 3, while the graph (D) reports hemoglobin content quantified by Drabkin reagent (n = 3 plugs). ***p < 0.001 vs VEGF alone.