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. 2018 Feb 3;9(17):13390–13406. doi: 10.18632/oncotarget.24385

Figure 1. The effects of amiodarone on target gene and protein expression in HeLa cells.

Figure 1

HeLa cells were treated with indicated amount of amiodarone for indicated time. The cells were collected and subjected to (A) RT-PCR analysis of SRSF3, Slu7, p53, cyclin D1, p21, ATF3, COX-2, and GAPDH (loading control) mRNA expression; (B) immunoblot analysis for the detection of SLU7, SRSF3, p53, cyclin D1, p21, ATF3, COX-2, PARP, and ACTN (loading control) protein expression. PCR bands (A) were quantified through pixel density scanning and evaluated by ImageJ software, version 1.44a (http://imagej.nih.gov/ij/). The results are representative of two independent experiments.