Figure 11. The effects of amiodarone on miR-224 and its target genes in HeLa cell.

HeLa cells were treated with lower concentration of amiodarone for 20 h. The cells were collected and subjected to (A) the miR analysis for miR-224 expression; (B) the RT-PCR analysis for caspase3, TNFAIP1, COX-2, and GAPDH (loading control); and (C) the Western blotting analysis using antibodies against COX-2 and ACTN (loading control). The results are representative of three independent experiments.