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. 2018 Feb 3;9(17):13390–13406. doi: 10.18632/oncotarget.24385

Figure 5. The regulatory mechanism of amiodarone on the SRSF3 expression.

Figure 5

HeLa cells were treated indicated amount amiodarone with pre-treated 2 h of vehicle, CHX (50 mg/ml), Act D (1 µM), and calcium chloride (1 mM) for 6 h. Cells were subject to the (A) Western blotting analysis and (B) RT-PCR analysis. The numerical data below each band is to indicate the ratio of specific mRNA (A) or protein (B) with control mRNA (GAPDH) or control protein (HuR) using the ImageJ software. The results (A and B) are representative of three independent experiments. (C and D) HeLa, GBM8401, U118MG cells were transiently transfected with 0.5 mg pGL3.SRSF3 (–1650/+171)-LUC and cells were treated with indicated amount of amiodarone for 20 h. Cells were harvested for luciferase reporter assay with the Promega Luciferase Assay Kit. The numbers (C and D) above the columns indicate the luciferase activity relative to an index of 1 for the reporter alone with vehicle.