Figure 4. F497L demonstrates sensitivity to imatinib and nilotinib in vitro.

(A) LD50^IM (B) LD50^NIL and (C) LD50^DAS were determined in KU812 control cells compared with KU812 10 μM asciminib cells (F497L mutation at 100%) in the absence and presence of dual ABCB1 (verapamil) and ABCG2 (Ko143) inhibition. Statistical analyses compared 1) control cells vs resistant cells in the absence of inhibition (asterisks) 2) resistant cells in the absence vs presence of ABCB1/ABCG2 inhibition (hashes) and 3) control cells in the absence of inhibition vs resistant cells in the presence of ABCB1/ABCG2 inhibition (carets). Data represent the mean of at least three independent assays. Analyses were performed using unpaired Student’s t-test (Welch’s correction was applied for data groups with unequal SD). Statistically significant p-values are denoted by carets (^), hashes (#) or asterisks (^*p < 0.05, ^**p < 0.01, ^***p < 0.001). Error bars represent SEM. Ver = verapamil.