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. 2018 Feb 6;9(17):13593–13611. doi: 10.18632/oncotarget.24423

Figure 3. In situ proximity ligation assay in rat hippocampus.

Figure 3

A2A-A2BAR-specific PLA clusters in the CA3 region of the dorsal hippocampus of the rat (Bregma: –3.6 mm). The sampled region is taken from the framed section of the dorsal hippocampus in the upper right corner of the figure. The microphotographs taken are based on 20 Z-scans (1 µm each). The nuclei are shown in blue. A high density of PLA positive clusters in red are visualized mainly in the pyramidal cell layer shown also in higher amplification in the panel at the lower right part of the figure. A few are indicated by arrows. The diameter range of the clusters is 0.5–2 µm. They are mainly located in a perisomatic position around the blue nuclei but also in the neuropil. A low density of specific PLA clusters is also found in the radiatum and oriens close to the pyramidal cell layer.