Table 3. Prediction of functional significance of the D16N NKX2.5 mutation by using multiple computational programs.

Name of software	D16N		Cut-off
	Prediction	Score
PMut	Pathological	0.72 (86%)	0.5
SIFT	Pathological	0	0.05
PROVEAN	Pathological	-3.75	-2.5
PANTHER	Pathological	1037	450
PHD-SNP	Pathological	0.674	0.5
PredictSNP	Pathological	0.72%	0.50%
PolyPhen-2	Benign	0.029	0.5