Figure 2. AT2R agonist supplementation suppressed the progression of prostate carcinogenesis and induced apoptosis in the TRAP model.
(A) Representative histological findings of VP and LP of TRAP. Representative histological findings of adenocarcinoma (arrow), HG-PIN (arrowhead), and LG-PIN (asterisk). (B, C) Labeling indices for Ki67- (B) and TUNEL- (C) positive cells in VP and LP. Hematoxylin was used as a nuclear counterstain. Data are presented in a box plot, n = 12 per group, *P < 0.05, **P < 0.01, ****P < 0.0001 vs. the control group. (D, E) A Western blotting analysis for Erk 1/2, phospho-Erk 1/2, p38 Mapk, phosphor-p38 Mapk, Cyclin D1, caspase 3 and 7, cleaved (cl)-caspase 3 and 7, and β-actin in VP (D) and LP (E) of TRAP. Western blotting was repeated at least three times, and each lane represents an individual rat (n = 4 per group).
