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. 2018 Feb 14;9(17):13884–13893. doi: 10.18632/oncotarget.24490

Table 2. Binding parameters of bsDbs with different domain orders.

	sEGFR				CD3εγ		sEGFR
	k_on^a (× 10⁴ M^–1s^–1)	k_off ^a (× 10^–3 s^–1)	K_A^a (× 10⁷ M^–1)	K_A^b (× 10⁷ M^–1)	K_A^b (× 10⁷ M^–1)		K_A^a (× 10⁷ M^–1)
hEx3-HL	9.3	2.0	4.6 ± 0.03	6.2 ± 0.12	1.7 ± 0.22	E2x3-HL	52.7 ± 1.48
hEx3-LH	7.3	3.6	2.0 ± 0.01	0.9 ± 0.19	1.0 ± 0.12	E2x3-LH	19.0 ± 0.33
hEx3-O5	2.4	3.7	0.6 ± 0.04	n.d.	0.6 ± 0.08	E2x3-O2	0.03 ± 14.92
hEx3-5O	15.2	3.4	4.5 ± 0.01	6.5 ± 0.66	n.d.	E2x3-2O	9.9 ± 0.13

^aValue evaluated by surface plasmon resonance spectroscopy.

^bValue evaluated by isothermal titration calorimetry.

n.d., not determined.