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Development of an assay to quantify NK cell-mediated cytotoxicity and ADCC in O-PDXs a Dissociated tumor cells from O-PDXs were plated for the ADCC assay. IL-2 activated or resting NK cells were added ± Hu14.18K322A (t = 0 h) and incubated for 12 h. The CellTiter-Glo luminescent cell viability assay was used to quantify ADCC. b ADCC results are plotted as relative light units (RLU) correlate with confluence in cells that are growing in two dimensions. RLU relative light units
