Fig. 1.

OrthomAb synthesis and purification. a Schematic depicting click conjugation of anti-CD134 and -CD137. b Non-reducing SDS–PAGE showing species at different stages of synthesis, with the two right-most lanes showing a representative unpurified reaction product mixture. Chromatographic tracing of A280 versus time (c) and SDS–PAGE (d) indicate the species present in each fraction. e Chromatographic tracings with SDS–PAGE image overlays depicting species present at each purification round for a representative triple-purified OrthomAb batch, with calculated yields shown at each step. f Representative SDS–PAGE showing purified OrthomAb dimer