Fig. 3.
OrthomAb boosts cytokine secretion by peptide-stimulated CD8 T cells. OT-I Rag KO splenocytes (1 × 105) were cultured 24 h with SIINFEKL (SFKL) peptide at 50 pg/ml (a) or the indicated concentrations (b) plus 2 µg/ml isotype control IgG, control conjugate or OrthomAb. a Supernatant IL-2 and IFN-γ measured by ELISA, mean ± SD, n = 2–4, representative of at least 3 independent, similar experiments. b Supernatant IL-2, IFN-γ, and IL-6 with the indicated SFKL peptide and OrthomAb concentrations