Fig. 4.

OrthomAb boosts cytokine secretion by peptide-stimulated CD8 T cells more effectively than anti-CD134 and anti-CD137. OT-I Rag KO splenocytes (1 × 10⁵) were cultured 48 h with SFKL peptide and costimulators at the indicated concentrations. a Supernatant IFN-γ, IL-6, and IL-17 with titrated concentrations of SFKL peptide and costimulators. b Supernatant IFN-γ, IL-6, and IL-17 pooled from the 30 pg/ml SFKL peptide plus 0.2, 0.6, and 2.0 µg/ml costimulator wells in (a). c CD25 expression on OT-I CD8⁺ T cells. Data are representative of multiple similar trials