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. Author manuscript; available in PMC: 2018 Aug 19.
Published in final edited form as: Nat Genet. 2018 Feb 19;50(3):460–471. doi: 10.1038/s41588-018-0054-7

Figure 6. TEDC1 and TEDC2 form a tetrameric complex with δ- and ε-tubulins.

Figure 6

a) IMCD3 cells stably expressing TEDC2-LAP were immunostained with antibodies to centrin2 and γ-tubulin to visualize centrioles. Scale bar: 5 μm (2 μm for insets). Representative images are shown for three independent experiments. b) Mass spectrometry analysis of TEDC2-associated proteins purified from IMCD3 cells reveals TEDC1, ε-tubulin, δ-tubulin in nearly stoichiometric amounts, as well as α/β-tubulin and CENPJ. For each protein, the normalized spectral abundance factor (NSAF), the percent of the protein covered by identified peptides, and the percentile rank of the corresponding gene in the screen dataset are indicated. c) Binding of TEDC1 and TEDC2 was assessed via co-immunoprecipitations performed in HEK293T cells transfected with plasmids encoding the indicated proteins. Recovered proteins were analyzed by Western blot. Representative blots are shown for two independent experiments. See also Supplementary Fig. 6b and Supplementary Fig. 8.