Fig. 1.

Murine TCR-engineered T cells expanded in NAC exhibit enhanced tumor control when transferred in vivo and maintain a DNA damage and AICD-resistant phenotype. a Tumor growth and b survival was determined in untreated mice (no cells, n = 9) or mice receiving TRP-1 TCR-transduced T cells that were expanded in the absence or the presence of NAC (n = 12). c, d Splenocytes and TILs were isolated from a subset of mice (n = 5) 9-day post-transfer and restimulated as described in “Materials and methods”. Vβ14⁺CD8⁺ TRP-1 TCR-transduced cells analyzed for c Annexin V staining and d γH2AX expression. e Granzyme B expression in Vβ14⁺CD8⁺ splenocytes. *p < 0.05; **p < 0.01; ****p < 0.0001