Fig. 2.

Human TIL1383I TCR-transduced T cells cultured in NAC have enhanced antioxidant capacity, reduced evidence of DNA damage, and superior cytotoxic activity against melanoma cells in vitro. TIL1383I TCR-transduced human T cells were collected on day 20 and CD34⁺CD8⁺ cells analyzed for surface thiols (a) and for γH2AX following co-culture with melanoma target cells (b). Representative histograms and quantification (mean ± SEM) are shown. c, d In vitro cytotoxicity assay as described in “Materials and methods”. c Representative contour plot of Annexin V & 7AAD expression in melanoma cells at a 2:1 E:T ratio (left) and quantification (mean ± SEM) of Annexin V⁺/7AAD⁺ cells over all E:T ratios (right). d Quantification (mean ± SEM) of Annexin V MFI of CD34⁺ gated cells used in “c”. e Representative histogram overlay and quantification (mean ± SEM) of granzyme B expression in CD34⁺ CD8⁺ cells after rapid expansion (± NAC). n = 5–6; *p < 0.05; **p < 0.01; ****p < 0.0001