Fig. 5.

Acute Treatment of T cells with NAC rapidly degrades Foxo1 in an Akt-dependent manner. T cells were pretreated with LY294002 (20 µm), AktX (5 µm), or MG132 (20 µm) for 60 min prior to adding NAC (25 mM) for an additional 60 min. Display is a representative histogram overlay of CD8⁺ cells. Similar results were obtained from four individual donors treated with NAC versus untreated controls (p = 0.0004)