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. 2018 Mar 15;9:373. doi: 10.3389/fimmu.2018.00373

Figure 1.

Figure 1

TRIB1 is overexpressed in B cells from quiescent systemic lupus erythematosus (SLE) patients. (A) TRIB1 mRNA expression levels in transcriptome analysis of purified mature B cells from 17 SLE quiescent patients (light gray) and from a subgroup of 5 patients (dark gray) compared to 9 age- and sex-matched healthy donors (HD). X-fold represents the 2exp(Pi-Tmean) value for the patients, where Pi is the value of the TRIB1 probe set signal for a given patient, and T mean the mean value of signals for the same probe set for the HD (Tmean = 8.005). (B) Quantitative real-time RT-PCR analysis of TRIB1 mRNA expression performed on purified B cells from HDs (n = 4) and quiescent SLE patients (n = 4). Relative expression levels were calculated with the comparative Ct method using the mean of the Ct between HPRT1, ACTB, and GAPDH for normalization. Each bar represents the level of TRIB1 mRNA relative to HD controls (Error bars, SD; *p < 0.05, ***p < 0.001, Mann and Whitney test).