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. 2018 Mar 15;9:373. doi: 10.3389/fimmu.2018.00373

Figure 5.

Figure 5

Trib1 overexpression in B cells negatively regulates Ig production in vitro. (A,B) Total splenocytes were collected from Trib1-ROSA and Trib1-ROSA Mb1Cre mice and stimulated with TLR ligands or a combination of LPS (TLR4 ligand) and IL-4 during 72 h in vitro. The concentration of total IgG (A) and IgG1 (B) in the supernatant was measured by ELISA. (C) Splenic mature B cells were purified from Trib1-ROSA and Trib1-ROSA Mb1Cre mice and stimulated in vitro with a combination of LPS and IL-4. The concentration of total IgG and IgG1 in the supernatant was measured by ELISA. (D) Total splenocytes from Trib1-ROSA and Trib1-ROSA Mb1Cre mice were stimulated with anti-CD40, IL-4, and IL-21 during 72 h in vitro. The concentration of total IgG in the supernatant was measured by ELISA. Each dot represents the result for one animal (US: unstimulated) (*p < 0.05, **p < 0.001, Mann and Whitney test).