Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Mar 15;9:373. doi: 10.3389/fimmu.2018.00373

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2018 Simoni, Delgado, Ruer-Laventie, Bouis, Soley, Heyer, Robert, Gies, Martin, Korganow, San Martin and Soulas-Sprauel.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Trib1 interacts with COP1 and CD72 in B cells. CH12 cells were transfected with pMX-PIE/Flag-tagged Trib1 or GFP. The samples were immunoprecipitated with an anti-Flag antibody to precipitate both Flag-tagged proteins from cell cytoplasmic lysates (IP anti-Flag) or with total mouse serum (Control). The result is representative of at least three independent experiments. Note: considering the differences in Flag protein expression in CH12-Trib1 and CH12-GFP cells, two different dilutions of anti-Flag antibody were used for the bottom left part (1:1,000) and the bottom right part (1:20,000). (Input, total protein lysate; -, empty well).