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. 2018 Mar 15;9:100. doi: 10.3389/fendo.2018.00100

Figure 3.

Figure 3

Validation of Rap1b as a direct target of miR-518b in human trophoblast cells. (A–D) Change in Rap1b expression upon miR-518b overexpression in human trophoblast cells. The HTR8/SVneo cells were transfected with miR-518b or scramble negative control (NC). The efficiency of transfection was shown in (A), and the expression of Rap1b was revealed by real-time PCR (B) and Western blotting (C,D). (E) Schematic diagram of the plasmid construction for dual-luciferase report assay. (F) Dual-luciferase reporter assay in HTR8/SVneo cells transfected with wild-type (WT) or mutated (MUT) reporter plasmid, and with miR-518b or scramble NC. Each of the tests was replicated for three times. Data are presented as mean ± SEM. * P < 0.05.